A novel method for rapid determination of risperidone in human plasma using Reversed-Phase Liquid Chromatography with Ultra-Violet detection
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Abstract
Aim: A simple and sensitive High-Performance Liquid Chromatographic (HPLC) method with UV detection is described for the quantitation of risperidone in human plasma, using bisoprolol as internal standard.
Methods: After sample alkalinization with 900 µl of NaOH, the test compounds were extracted from plasma using diethylether. The tubes were centrifuged twice and an evaporation to dryness under a gently stream of nitrogen at 50°C (~10 min) was done. 50 μl of the solution was injected into a C18 Nucleosyl analytical column (5 μm, 150×4.6 mm I.D). The mobile phase consisted of phosphate buffer (10 mM, pH 4.9 and 30% acetonitrile, (70:30, v/v), and was delivered at a flowrate of 1.0 ml/min. Results: The peaks were detected using a UV detector set at 279 nm and the total time for a chromatographic separation was about 8 min. This new method was validated for the concentration range 5–150 ng/ml. Mean recoveries were 92 % for risperidone. Intra- and inter-day relative standard deviations were less than 15% for our compound, while accuracy, expressed as percent error, ranged from 0.13 to 19 %. The limit of quantitation (LLOQ) was 3.23 ng/ml for the analyte.
Conclusion: The method shows good specificity with respect to commonly prescribed psychotropic drugs.
Keywords:
Risperidone, HPLC, Bisoprolol, Therapeutic drug monitoring, Pharmacokinetics##plugins.themes.academic_pro.article.details##
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