The effects of high-intensity interval training and iranian propolis extract on serum levels of TRPV4 and CYP2E1 proteins in patients with nonalcoholic fatty liver


Khadijeh Irandoust
Mahsa Gholamhosseini
Rasool Samimi
Ismail Dergaa
Helmi Ben Saad
Mortez Taher


Introduction. Non-alcoholic fatty liver disease (NAFLD) is the most common cause of liver failure, fibrosis, cirrhosis, and liver cancer, which can eventually lead to death.

Aim. To investigate the effects of high-intensity interval training (HIIT) and iranian propolis extract on serum levels of transient receptor potential cation channel subfamily V member 4 (TRPV4) and cytochrome P450 2E1 (CYP2E1) proteins in patients with NAFLD.

Methods. Thirty-two patients with NAFLD (mean±standard deviation of age: 45.1±3.6 years; body mass index: 30.0±3.6 kg/m2) were assigned in a randomized control trial to one of the following groups: HIIT (n=8), propolis supplement (n=8), propolis + HIIT (n=8), and controls (n=8). The subjects participated in eight weeks of HIIT (one bout of 1-min intervals at 80-95% of the maximal heart-rate, interspersed by two min at 50-55% of the reserve heart-rate). The Propolis supplement was taken three times a day by the patients in the form of 50 mg tablet after the main meals. Body composition, liver injury test (eg; Alanine- and Aspartate- aminotransferase levels), liver ultrasound and serum levels of TRPV4 and CYP2E1 were measured before and after intervention. One-way analysis of variance was used to compare post-tests among the groups.

Results. HIIT significantly reduced serum levels of TRPV4 protein (p=0.001). The reduction in CYP2E1 was not significant in HIIT group (p=0.075). Propolis consumption had no significant effect on serum levels of CYP2E1 protein (p=0.059), and TRPV4 (p=0.072). There was a significant decrease in TRPV4 and CYP2E1 in the HIIT (p=0.001) .... (abstract truncated at 250 words).


ALT, AST, Liver enzymes, Oxidative stress, Physical activity , Sports, Visceral fat


Author Biography

Helmi Ben Saad, Physiology Laboratory, Faculty of Medicine of Sousse , University of Sousse. Heart Failure (LR12SP09) Research Laboratory, Farhat HACHED Hospital, Sousse, Tunisia.

  • Professor of Physiology and Functional Explorations at the Faculty of Medicine of Sousse - Tunisia, Department of Physiology and Functional Explorations at EPS Farhat HACHED - Sousse - Tunisia.
  • President of the College of Physiology and Functional Explorations (terms: 2014-2017; 2021-2023).
  • Master's degree in Biological and Medical Sciences from the University of Montpellier I, France.
  • World expert in spirometry (ranked 1st in Africa):
  • Diploma of Advanced Studies in "Human Movement Sciences" from the University of Montpellier I, France.
  • Interuniversity Diploma "Pathophysiology of Exercise and Functional Exercise Explorations" from the University of Montpellier 1, France.
  • International PhD in Sciences between the Universities of Montpellier I (France) and Monastir (Tunisia).
  • European Spirometry Driving License Part 1 (theory only) and the European Spirometry Training Program (Part 2: knowledge and competence in practice) and the European Spirometry train-the-trainer course.
  • Invited speaker at numerous international congresses on respiratory functional explorations.
  • Author of over 220 scientific articles in indexed and impact factor journals.
  • Potential reviewer for over 80 indexed and impact factor journals and potential external reviewer for the University of Cape Town (South Africa) and Mutah (Jordan).
  • Winner of the Sadok BESROUR Award for Excellence in Medical Research (2021).
  • Web:


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