Animal model of liver ischemia reperfusion: biochemical and histological evaluation

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Published Aug 22, 2016
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Vol. 94 No. 3 (2016): Issue Mar 2016

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Henda Ferchichi
Sarra Bacha
Nadia Kourda
Emna Gaies
salma Melaouhia
Mohamed Lakhal
Anis Klouz
Sameh Trabelsi
Issam Salouage

Abstract

Abstract:
Introduction:
During last years, significant progress was made in the comprehension of the hepatic lesions after Ischemia-Reperfusion episode in order to improve the results in practice clinical.
Aim:
To avoid or reduce the damage induced by Ischemia-Reperfusion, we developed a model of hepatic Ischemia-Reperfusion with variable periods of reperfusion from 0 to 24 hours. 
Methods:
Our study related to rats Wistar males.  Six various groups were studied: the first citationsRaw group (without neither ischemia and reperfusion), the second group with ischemia of 90 min and without reperfusion and the 3end , 4end, 5end and 6end groups in addition to ischemia, underwent a reperfusion of 30 min, 2h, 6h and 24h respectively. 
The damage of hepatic Ischemia-Reperfusion was evaluated by a biochemical test based on the proportioning of transaminases and an anatomopathologic study by optical microscopy for the determination of the degree of hepatic attack. 
Results:
The results obtained seem to show an aggravation of the liver lesions and an increase in the plasmatic rates of AST and ALT in relation with the duration of the reperfusion.  Indeed, the maximum of damage was observed after 2 hours of reperfusion. We observed a reduction in the lesions after 6h and 24h of reperfusion. 
Conclusion:
Our work enabled us to describe a simple model of hepatic Ischemia-Reperfusion with functional, biochemical and anatomopathologic tests.

Keywords:

Ischemia, reperfusion, apoptosis, necrosis, transaminases.

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