Contribution of Polymerase Chain Reaction for detection of malaria in Tunisia.

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Published Jan 21, 2016
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Vol. 93 No. 12 (2015): Issue Dec 2015

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Emna Siala
Rym Essid
Marwa Smiri
Amira Doggui
Ines Ben Sghaier
Karim Aoun
Aida Bouratbine

Abstract

SUMMARY
Background: In Tunisia, detection of Plasmodium in asymptomatic individuals from endemic countries is a critical measure in national program of malaria eradication. The screening is based on microscopic examination of thick and thin blood smears. However, the performance of this diagnosis is closely related to the experience of biologist and the parasitaemia.
Aim : The objective of this study was to evaluate the contribution of the PCR in the screening of malaria.
Methods : This prospective study involved 260 students from malaria endemic areas who were screened for malaria between september 2011 and june 2013. Each subject had a blood sample which was examined for malaria by microscopy and nested multiplex PCR.
Results : PCR detected the presence of Plasmodium in 13 blood samples (5%). While microscopy was positive only in nine cases (3.5%). The discordances involved five negative samples at microscopy and which were positive in PCR and a negative sample in PCR which was positive at microscopy. A mixed infection with Plasmodium falciparum and Plasmodium malariae was identified by PCR. For this case, microscopy diagnosed only Plasmodium falciparum specie.
Conclusion : PCR is more efficient than microscopy in detecting low parasitaemia ; particularly observed in asymptomatic subjects. This technique allows to reduce asymptomatic carriage of Plasmodium and reduce the risk of a resumption of transmission of malaria in our country.

Keywords:

Malaria, screening, thin blood smear, thick blood smear, PCR, Tunisia.

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References

  1. Chadli A, Kennoun MF, Kooli J. Le paludisme en Tunisie: Historique et état actuel. Bull Soc Pathol Exot 1985; 78:844-51.
  2. Chahed MK, Bouratbine A, Krida G, Ben Hamida A. Réceptivité de la Tunisie au paludisme après son éradication: analyse de la situation pour une adéquation de la surveillance. Bull Soc Pathol Exot 2001; 94:271-6.
  3. Bouratbine A, Chahed Mk, Aoun K, Krida G, Ayari S, Ben Ismail R. Imported malaria in Tunisia. Bull Soc Pathol Exot 1998; 3:203-7.
  4. Delaunay P, Estran-Pomares C, Marty P. Diagnostic du paludisme : frottis sanguin, goutte épaisse et tests antigéniques. Med et Mal Infect 2008; 38:121-3.
  5. De Gentile L, Geneviève F. Le paludisme d'importation: diagnostic au laboratoire. Rev Fr Lab 2000; 321:25-9.
  6. Berry A, Iriat X, Magnaval JF. Nouvelles méthodes de diagnostic du paludisme. Rev Franc Lab 2009; 416:65-70.
  7. OMS. Basic malaria microscopy: Part I Learner' guide; Part II tutor's guide. World Health Organization, Geneva 1991.
  8. Snounou G, Viriyakosol S, Zhu XP, et al. High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction. Mol Biochem Parasitol 1993; 61:315-20.
  9. Berry A, Fabre R, Benoit-Vical F, Cassaing S, Magnaval JF. Contribution of PCR-based methods to diagnosis and management of imported malaria. Med Trop 2005; 65:176-83.
  10. Brown AE, Kain KC, Pipithkul J, Webster HK. Demonstration by the polymerase chain reaction of mixed Plasmodium falciparum and Plasmodium vivax infections undetected by conventional microscopy. Trans R Soc Trop Med Hyg 1992; 86:609-12.
  11. Snounou G. Detection and identification of the four malaria parasite species infecting humans by PCR amplification. Methods Mol Biol 1996; 50:263-91.
  12. Proux S, Suwanarusk R, Barends M, et al. Considerations on the use of nucleic acid-based amplification for malaria parasite detection. Malar J 2011; 10:323.
  13. Vivek J, Muralidhar V, Sudha V, Alvin M. Comparison of the clinical profile and complications of mixed Malarial infections of Plasmodium falciparum and Plasmodium vivax versus Plasmodium falciparum mono-infection. Sultan Qaboos Univ Med J 2001; 11(3):377-82.
  14. Aoun K, Siala E, Tchibkere D, et al. Paludisme d'importation en Tunisie: conséquences sur le risque de réintroduction de la maladie. Med Trop 2010; 70:33-7.
  15. Ghazi K, Rhaiem A, Bouatour A. Effet de la qualité des eaux sur l'expression du potentiel biotique du moustique Culex pipiens dans la région de Ben Arous. Bulletin de la société entomologique de France 1997; 102:143-50.
  16. Bouattour A, Rhaiem A, Bach Hamda D. Etude de la capacité vectorielle d'anophèles labranchiae dans la région de Nefza. Rapport du programme de recherche de l'Institut Pasteur de Tunis, soutenu par la coopération française et Agence National de la protection de l'environnement 1993; 1:54-60.
  17. Baldari M, Tamburro A, Sabatinelli G, et al. Malaria in Maremma, Italy.Lancet 1998; 351:1246-7.
  18. Maldonaldo Y A, Nahlen BL, Roberto RR, et al. Transmission of Plasmodium vivax malaria in San Diego County, California, 1986. Am J Trop Med Hyg 1990; 42(1):3-9.
  19. 19 Mantel CF , Klose C, Scheurer S, Vogel R, Wesirow AL, Bienzle U. Plasmodium falciparum malaria acquired in Berlin, Germany. Lancet 1995; 346:320-1.
  20. Ciceron L, Jaureguiberry G, Gay F, Danis F. Development of a Plasmodium PCR for monitoring efficacy of antimalarial treatment. J Clin Microbiol 1999; 37:35-8.Tableau 1 : Séquences des amorces utilisées pour la PCR spécifique des espèces plasmodiales