Detection of PEr 1 Extended-spectrum β-Lactamase among Nosocomial Providencia stuartii isolates in Tunisia

##plugins.themes.academic_pro.article.sidebar##

Published Aug 14, 2014
Download
pdf
Vol. 92 No. 4 (2014): Issue Apr 2014

##plugins.themes.academic_pro.article.main##

Hella Lahlaoui
Mohamed Ben Moussa

Abstract

Background: The Emergence of extended-spectrum beta-lactamases (ESBLs) in Enterobacteriace species imparting resistance to thirdgeneration cephalosporins is a growing concern worldwide.
aims: This study was undertaken to characterize ESBL producers from clinical isolates of Providencia stuartii in Tunisia.
methods: We analysed ten non-duplicate ESBL positive, P. stuartii isolates collected in intensive care units of military Hospital in Tunisia from blood specimens and presented an extended-spectrum resistance phenotype . the presence of the blaPER-1 encoding genes was confirmed by polymerase chain reaction.
results:We detected PER-1 in three of ten isolates of P. stuartii. The gene coding for this enzyme was located on a 100 kb conjugative plasmid that encoded a β-lactamase with a pI of 5.3.
Conclusion: This study suggests the spreading of this gene among P. stuartii. clinical strains in the next year in Tunisia.

Keywords:

Extended spectrum β-lactamases; Providencia stuartii; PER-1; Tunisia

##plugins.themes.academic_pro.article.details##

References

  1. Poirel L, Bruderer T, Frei R, Bernabeu S, Graber P, Nordmann P. Multidrug-resistant Providencia stuartii expressing extended-spectrum β- lactamase PER-1, originating in Kosovo. J Antimicrob. Chemother. 2008; 61(6): 1392-1393.
  2. Bradford PA. Extended-spectrum β-lactamases in the 21st century: characterization, epidemiology and detection of this important resistance threat. Clin Microbiol Rev. 2001; 14(4):933-951.
  3. Poirel L, Menuteau O, Agoli N, Cattoen C, Nordmann P. Outbreak of extended-spectrum β-lactamase VEB-1-producing isolates of Acinetobacter baumannii in a French hospital. J Clin Microbiol. 2003; 41(8): 3542-3547.
  4. Tsou MF, Yu WL, Hung SW, Chi CJ, Chieh, J, Chen SC, Wu LT. Detection of cefotaxime-resistant CTX-M-3 in clinical isolates of Serratia marcescens. Folia Microbiol. 2004; 49(4): 452-456 .
  5. Eraç B, Gülay Z. Molecular Epidemiology of PER-1 Extended Spectrum β- Lactamase among Gram-negative Bacteria Isolated at a Tertiary Care Hospital. Folia Microbiol. 2007; 52(4):535-41.
  6. Naas, T, Poirel L, Nordmann P. Minor extended-spectrum β-lactamases. Clin Microbiol Infect. 2008; 1(14): 42-52.
  7. Bauernfeind A, Stemplinger I, Jungwirth R, Mangold P, Amann S, Akalin E, Ang O, Bal C, Casellas JM. Characterization of beta-lactamase gene blaPER-2, which encodes an extended-spectrum class A β-lactamase. Antimicrob Agents Chemother. 1996; 40(4): 616-62.
  8. Vahaboglü H, Dodanli S, Eroglu C, Ozturk R, Soyletir G, Yildirim I, Avkan V. Characterization of multiple antibiotic-resistant Salmonella typhimurium strains: molecular epidemiology of PER-1-producing isolates and evidence for nosocomial plasmid exchange by a clone. J Clin Microbiol. 1996; 34(12): 2942-2946.
  9. Bahar G, Erac B, Mert A, Gulay Z. PER-1 production in a urinary isolate of Providencia rettgeri. J Chemother. 2004; 16(4): 343-346
  10. Pagani, L, Mantengoli E, Miglivacca R, Nucleo E, Pollinis S, Spalla M, Daturi R, Romero E, Rossolini GM. Multifocal detection of multidrugresistant Pseudomonas aeruginosa producing the PER-1 extended-spectrum β-lactamase in northern Italy. J Clin Microbiol. 2004; 42(6):2523-2529.
  11. Poirel L, Cabanne L, Vahaboglu H, Nordmann P. Genetic environment and expression of the extended-spectrum β-lactamase blaPER-1 gene in Gramnegative bacteria. Antimicrob Agents Chemother. 2005; 49(5): 1708- 1713.
  12. Danel F, Hall LMC, Gur D, Akalin HE, Livermore DM. Transferable production of PER-1 β-lactamase in Pseudomonas aeruginosa. J Antimicrob Chemother. 1995; 35(2): 281-294 .
  13. Mantengoli E, Rossolini GM. Tn5393d, a complex Tn5393 derivative carrying the PER-1 extended-spectrum β-lactamase gene and other resistance determinants. Antimicrob Agents Chemother. 2005 ;49(8): 3289-3296.
  14. Poirel L, Weldhagen GF, De Champs C, Naas T, Dove MG, Nordmann P. Characterization of the class A β-lactamase GES-2 from Pseudomonas aeruginosa with increased hydrolysis of imipenem. Antimicrob Agents Chemother. 2002; 49(1): 561-565.
  15. Barroso H, Freitas-Vieira A, Lito LM, Melo-Cristino J, Salgado MJ, Ferreira Neto H, Sousa JC, Soveral G, Moura T, Duarte A. Survey of Klebsiella pneumoniae producing extended-spectrum β-lactamases at a Portuguese hospital: TEM-10 as the endemic enzyme. J Antimicrob Chemother. 2000 ;45 (5): 611-616.
  16. Clinical and Laboratory Standards Institute: Approved standart M2-A8 and M7-A6. Performance standards for antimicrobial susceptibility testing. Clinical and Laboratory Standards Institute, Wayne, Pa.2009
  17. Ben-Hamouda T, Foulon T, Ben-Mahrez K. Involvement of SHV-12 and SHV-2a encoding plasmids in outbreak of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in a Tunisian neonatal ward. Microb Drug Resist. 2004; 10(2): 132-138.
  18. Perret CJ. Iodemetric assay of penicillinases. Nature. 1954; 174: 1012- 1013.
  19. Birnboim HC, Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979; 7(6): 1513-1523.
  20. Lee KM, Yoo JI, Yoo YS, Chung GT, Ahn TI, Lee YS. Investigation of Klebsiella pneumoniae isolates producing SHV-12 and SHV-11 betalactamases in Korean hospitals. J Microbiol Biotechnol. 2009; 19(9):1065-9.
  21. Chapman PA, Ellin M, Ashton R, Shafique W. Comparison of culture, PCR and immunoassays for detecting Escherichia coli O157 following enrichment culture and immunomagnetic separation performed on naturally contaminated raw meat products. Int J Food Microbiol. 2001; 68(16-2): 11-20.
  22. Lee W, Kang HY, Shin KS, Kim J. Multidrug-resistant Providencia isolates carrying blaPER-1, blaVIM-2, and armA. J Microbiol. 2007; 45(3): 272-274.
  23. Nordmann P, Ronco E, Nass T, Duport C, Michel-Briand Y, Labia, R. Characterization of a novel extended-spectrum β-lactamase from Pseudomonas aeruginosa. Antimicrob Agents. Chemother. 1993; 37(5): 962-969.
  24. Vahaboglu, H, Hall LMC, Mulazimoglu L, Dodanli S, Yıl-dırım I, Livermore D. Resistance to extended-spectrum cephalosporins caused by PER-1 β-lactamase, in Salmonella typhimurium from Istanbul. J Med Microbiol. 1995; 43(4): 294-299.
  25. Paterson DL, Hujer KM, Hujer AM, Yeiser B, Bonomo MD, Rice LB, Bonomo RA. Extended-spectrum β-lactamases in Klebsiella pneumoniae blood stream isolates from seven countries: dominance and widespread prevalence of SHV- and CTX-M-type β-lactamases. Antimicrob Agents Chemother. 2003; 47(11): 3554-3560.
  26. Empel J, Filczak K, Mrowka A, Hryniewicz, W, Livermore DM, Gniadkowski M. Outbreak of Pseudomonas aeruginosa infections with PER-1 extended-spectrum β-lactamase in Warsaw, Poland: further evidence for an international clonal complex. J Clin Microbiol. 2007; 45(9): 2829-2834.
  27. Poirel L, Naas T, Nordmann P. Genetic support of extended-spectrum beta-lactamases. Clin Microbiol Infect. 2008; 14 Suppl: 1: 75-81.