Multispecific Anti Neutrophil Cytoplasm Antibodies: A case report

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Published Sep 16, 2011
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Vol. 89 No. 9 (2011): Issue Sep 2011

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Marouane Haddad
Meherzia Bourguiba
Najla Ghrairi
Hayet Bouakkez
Ibtissem Nahdi
Awatef Dahmouni
Ikram Drira
Sadok Yalaoui

Abstract

Aim : To report the presence af ANCA with an unusual polyreactivity.
Case report : 50 year-old woman with pulmonary fibrosis whose immunological investigations showed the presence of ANCA with an unusual polyreactivity against several neutrophil proteins (PR3,MPO, BPI, lysozyme, elastase and cathepsine G) which could be related to a polyclonal hypergammaglobulinemia occurring in this
patient.
Conclusion : The international consensus on the testing of ANCA recommends seeking major specificities like MPO and PR3 which are good markers of ANCA-associated vasculitides. The use of multiantigenic ELISA can be helpful to detect various target antigens at the same time and may thus explain some atypical fluorescent patterns observed when searching for ANCA by Indirect immunofluorescence, these results, however, must be interpreted with caution.

Keywords:

Anti Neutrophil Cytoplasm Antibodies. Indirect Immuno Fluorescence Enzym Linked ImmunoSorbent Assay. Specificity

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