Is the rapid whole blood test useful for diagnosis and monitoring celiac disease in children?

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Published Jan 12, 2011
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Vol. 89 No. 1 (2011): Issue Jan 2011

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Lilia Laadhar
Maryam Kallel- Sellami
Mondher Zitouni
Ahmed Mehrezi
Sondes Makni
Mongi Ben Hariz

Abstract

Aim: To evaluate a new whole blood rapid test for the detection of IgA anti-transglutaminase (ATG) for diagnosis and diet survey of celiac disease (CD).
Methods: 57 children, 20 of them were CD patients on a gluten-free diet and 37 were under suspicion of CD were enrolled. IgAATG was detected by the conventional ELISA test and the new rapid whole blood test.
Results: Concordance between the 2 tests was 96.4%. All patients positive with ELISA were also positive by the rapid test. Only 2 patients were slightly positive by the rapid test and negative by ELISA.
Conclusion: Whole blood rapid test seems to be as performant as ELISA test for IgAATG detection.

Keywords:

Celiac disease, tissue transglutaminase, whole blood, rapid test

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References

  1. Dieterich W, Ehnis T, Bauer M, et al. Identification of tissue transglutaminase as the autoantigen of celiac disease. Nat Med 1997; 3:797-801.
  2. Maki M, Korponay-Szabo IR. Methods and means for detecting gluten-induced disease. Patent application PCT/FI02/00340, international publication number W002/086509 A19.
  3. Korponay-Szabo IR, Raivio T, et al. Celiac disease case finding and diet monitoring by point of care testing. Aliment Pharmacol Ther 2005; 22:729-37.
  4. Raivio T, Kaukinen K, Nemes E, et al. Self transglutaminase-based rapid celiac disease antibody detection by a lateral flow method. Alim Pharmacol Ther 2006; 24: 147-54.
  5. Nemec G, Ventura A, Stefano M, et al. Looking for celiac disease: diagnostic accuracy of two rapid commercial assays. Am J Gastroenterol 2006; 101:1597-600.
  6. Raivio T, Korponay-Szabo I, Collin P, et al. Performance of a new rapid whole blood celiac test in adult patients with low prevalence of endomysial antibodies. Dig Liv Dis 2007; 39:1057-63.
  7. Korponay-Szabo IR, Szabados K, Pusztai J, et al. Population screening for celiac disease in primary care by district nurses using a rapid antibody test: diagnostic accuracy and feasibility study. BMJ 2007; 335:1244-7.