Comparison of differents molecular methods for detection dna of human cytomegalovirus infection for immunodepressed

##plugins.themes.academic_pro.article.sidebar##

Published Nov 29, 2010
Download
pdf
Vol. 88 No. 12 (2010): Issue Dec 2010

##plugins.themes.academic_pro.article.main##

Leila Mhiri
Amine Slim

Abstract

Background: A Cytomegalovirus infection (HCMV) causes severe complications in immunosuppressed individuals (transplant recipients and AIDS patients).
Aim: To detect the DNA of the HCMV by three molecular methods, and to identify the fastest method and most significant.
Methods: we tested 50 samples in order to detect the presence of the HCMV. This research was carried out by molecular Hybridization, the pp65 Antigenemia and PCR on the blood of the patients presenting an infection to CMV.
Results: Molecular hybridization is positive for 64%, Antigenemia is detected in 26 cases (50%) and the plasmatic PCR is positive in 13 cases (26%). These studies demonstrated that molecular hybridization permitted CMV detection of different biological liquid but Antigenemia and PCR techniques were used to determine of from leukocytes. Plasma-PCR and Hybridization assay presented the qualitatifs results.
Conclusion: These studies indicate that there is a combining virological between molecular methods

Keywords:

Human Cytomegalovirus, Molecular hybridization, pp65 Antigenemia, PCR, immunosuppressed

##plugins.themes.academic_pro.article.details##

References

  1. Britt W.J., Alford C.A. Cytomegalovirus. In : Fields B. N., Knipe D. M., Howley P. M., eds. Virology, pp. 2493-2525 Philadelphia : Lippincott-Raven, 1996.
  2. Boivin G., Relanger R., Delagage R., Beliveau C., Demeurs C., Goyette N. Quantitative analysis of CMV viremia using the pp65 antigenemia assay and the Cobas Amplicor CMV Monitor PCR Test after blood and marrow allogenic transplantation. J. Clin. Microbiol 2000; 38: 4356-60.
  3. Huang E.S.- Detection of human cytomegalovirus and analysis of strain variation. J. Biol. Med 1976; 49: 29-43.
  4. Lisbay G., Dessau R.B., Andersen C.B., Ladeforged S. Polymerase chain as a rapid diagnostic assay for CMV infection in rénal transplant patients. APMIS 1994; 102: 690-94.
  5. Mazulli T., Wood S., Chua R., Walmsley .- Evaluation of the Digene Hybrid Capture System for detection and quantitation of human cytomegalovirus viremia in human immunodeficiency virus- infected patients. J. Clin. Microbiol 1996; 34: 2959-62.
  6. Meyerson D., Hachman R.C., Meyers J.D. Diagnosis of cytomegalovirus pneumonia by in situ hybridation. J. Infect. Dis 1984; 150: 272-77.
  7. Stephen.K., Fu-keung.K., Lai, T chan. Comparison of brite turbo assay and the digene hybrid capture CMV DNA (version 2.0) assay for quantification of CMV in renal transplant recipients. J. Clin. Microbiol 2000; 38: 3743-45.
  8. Stephen K., Chi yen Lo., Ignatus K.P., Tak Maochan.- Rapid CMV pp65 antigenemia assay by direct erythrocytes lysis and immunofluorescence staining. J. Clin. Microbiol 1998; 36: 638- 40.
  9. The T.H., Langenhuysen M.M. Antibody reactions to virusspecific early antigens (EA) in patients with cytomégalovirus (CMV) infection. Clin. Exp. Immunol 1974; 16: 1-12.
  10. The T.H., Van der Bij W., Van der Berg A.P., Van der Giessen M. Cytomegalovirus antigenemia. Rev. Infect. Dis 1990; 12: S737- S744.
  11. Van der Bij W., Schirm J., Torensm R.- Comparison between viremia and antigenemia for detection of cytomegalovirus in blood. J. Clin. Microbiol 1988; 26: 2531-35.
  12. Veal N., Payan C., Fray D., et al. Novel DNA assay for cytomegalovirus detection: comparaison with conventional culture an pp65 antigenemia assay. J. Clin. Microbiol 1996 ; 34 : 3097-100.
  13. Wattré P., Dewilde A., Lobert P.E., Actualités sur la pathologie du cytomégalovirus humain, carrefour des spécialités. Rev. Med. Interne 1995 ; 16 : 354-67.