La tunisie Medicale - 2022 ; Vol 100 ( n°010 ) : 697-705
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Objective: To evaluate the effect of six weeks of high intensity interval training (HIIT) and L-Arginine supplementation on interleukin-6 (IL-6) levels and body composition in Iranian adult trained males.
Methods: This experimental study was performed as an intervention with a pretest-posttest design in three experimental groups and one control group. Fortyeight young males from Qazvin province (Iran) were selected voluntarily based on convenience sampling. Participants were randomly divided into four groups (12 participants in each group): “HIIT”; “L-Arginine supplementation”; “HIIT + L-Arginine supplementation”, and “HIIT + placebo”. At 7 a.m., when the level of inflammation was at its lowest, a blood sample was taken from the participants, and body mass index (BMI), body fat percentage (BFP), and lean body mass (LBM) were determined. IL-6 analysis was performed using STATE FAX device and ELISA method. Training sessions were conducted for six consecutive weeks, three sessions a week. Analysis of covariance and Bonferroni post hoc test were used to analyze the data.
Results: i) There were no significant differences between groups in BMI, BFP, or LBM. ii) There was a significant difference in IL-6 levels between the groups (p < 0.05), so that the inflammatory levels in the “HIIT + L-Arginine supplementation” and “HIIT + placebo” groups were lesser than the “HIIT” (0.002 and <0.001, respectively) and “L-Arginine supplementation” (<0.001and <0.001, respectively) groups. HIIT “seems” to reduce the level of inflammation.
Conclusion: HIIT had no significant effect on body composition indices. Plasma IL-6 levels decreased after six weeks of HIIT and L-Arginine supplementation. The level of IL-6 in the “HIIT + L-Arginine supplementation” and “HIIT + placebo” groups were lower than the control group (i.e.; “HIIT”) and supplement control group (i.e.; “L-Arginine supplementation”).

Key - Words
  1. Kohut M, McCann D, Russell D, Konopka D, Cunnick J, Franke W, et al. Aerobic exercise, but not flexibility/resistance exercise, reduces serum IL-18, CRP, and IL-6 independent of β-blockers, BMI, and psychosocial factors in older adults. Brain Behav Immun. 2006;20(3):201-9.
  2. Rehman K, Akash MSH, Liaqat A, Kamal S, Qadir MI, Rasul A. Role of interleukin-6 in development of insulin resistance and type 2 diabetes mellitus. Crit Rev Eukaryot Gene Expr. 2017;27(3):229-36.
  3. Wagenmakers AJ, Pedersen BK. The anti-inflammatory effect of exercise: its role in diabetes and cardiovascular disease control. Essays Biochem. 2006;42:105-17.
  4. Fernandez-Real JM, Broch M, Vendrell J, Gutierrez C, Casamitjana R, Pugeat M, et al. Interleukin-6 gene polymorphism and insulin sensitivity. Diabetes. 2000;49(3):517-20.
  5. Bastard JP, Jardel C, Bruckert E, Blondy P, Capeau J, Laville M, et al. Elevated levels of interleukin 6 are reduced in serum and subcutaneous adipose tissue of obese women after weight loss. J Clin Endocrinol Metab. 2000;85(9):3338-42.
  6. Esposito K, Pontillo A, Di Palo C, Giugliano G, Masella M, Marfella R, et al. Effect of weight loss and lifestyle changes on vascular inflammatory markers in obese women: a randomized trial. JAMA. 2003;289(14):1799-804.
  7. Thorand B, Baumert J, Doring A, Herder C, Kolb H, Rathmann W, et al. Sex differences in the relation of body composition to markers of inflammation. Atherosclerosis. 2006;184(1):216-24.
  8. Candrawati S, Huriyati E, Sofro ZM, Rujito L, Hidayah C, Hayuningtyas DA, et al. The effect of UCP2 45bp insersi/delesi genetic variation on the body composition of woman with obesity in continuous training and high-intensity interval training: A randomized controlled trial study. Ann Appl Sport Sci. 2022;10(2):0-.
  9. Amirsasan R, Nabilpour M, Pourraze H, Curby DJIJoSSfH. Effect of 8-week resistance training with creatine supplementation on body composition and physical fitness indexes in male futsal players. Int J Sport Stud Hlth. 2018; 1(3):e83810.
  10. Tayebi SM, Saeidi A, Fashi M, Pouya S, Khosravi A, Shirvani H, et al. Plasma retinol-binding protein-4 and tumor necrosis factoralpha are reduced in postmenopausal women after combination of different intensities of circuit resistance training and Zataria supplementation. Sport Sci Health. 2019;15(3):551-8
  11. Irandoust K, Taheri M, Mirmoezzi M, H’Mida C, Chtourou H, Trabelsi K, et al. The Effect of Aquatic Exercise on Postural Mobility of Healthy Older Adults with Endomorphic Somatotype. Int J Environ Res Public Health. 2019;16(22):4387.
  12. Martins FM, de Paula Souza A, Nunes PRP, Michelin MA, Murta EFC, Resende EAMR, et al. High-intensity body weight training is comparable to combined training in changes in muscle mass, physical performance, inflammatory markers and metabolic health in postmenopausal women at high risk for type 2 diabetes mellitus: a randomized controlled clinical trial. Exp Gerontol. 2018;107:108-115.
  13. Calle MC, Fernandez ML. Effects of resistance training on the inflammatory response. Nutr Res Pract. 2010;4(4):259-69.
  14. Kasapis C, Thompson PD. The effects of physical activity on serum C-reactive protein and inflammatory markers: a systematic review. J Am Coll Cardiol. 2005;45(10):1563-9.
  15. Pedersen BK. IL-6 signalling in exercise and disease. Biochem Soc Trans. 2007;35(Pt 5):1295-7.
  16. Cabral-Santos C, Castrillón CI, Miranda RA, Monteiro PA, Inoue DS, Campos EZ, et al. Inflammatory cytokines and BDNF response to high-intensity intermittent exercise: effect the exercise volume. Front Physiol. 2016;7:509.
  17. Das UN. Anti-inflammatory nature of exercise. Nutrition. 2004;20(3):323-6.
  18. Cullen T, Thomas AW, Webb R, Hughes MG. Interleukin-6 and associated cytokine responses to an acute bout of highintensity interval exercise: the effect of exercise intensity and volume. Appl Physiol Nutr Metab. 2016;41(8):803-8.
  19. Irandoust K, Ben Saad H, Mohammadgholiha F, Taheri M, Dergaa I. Effects of dry cupping therapy and creatine supplementation on inflammatory and cardiovascular responses to the Wingate test in handball players. Tunis Med. 2022;100(3):262-9.
  20. Jahani M, Nabilpour M, Campillo R R. Effects of L-arginine supplementation and aerobic training on hemodynamic indices of obese men. Int J Sport Stud Hlth.2(1):e88017
  21. Zargani M, Rahimi A, Mazaheri Tirani Z, Arabzadeh E, Feizolahi F. Swimming exercise and nano-l-arginine supplementation improve oxidative capacity and some autophagy-related genes in the soleus muscle of aging rats. Gene. 2023;850:146955
  22. Zhang H, Zheng Y, Zha X, Liu X, Ma Y, Loor JJ, et al. Dietary N-carbamylglutamate and L-arginine supplementation improves redox status and suppresses apoptosis in the colon of intrauterine growth-retarded suckling lambs. Anim Nutr. 2022;11:359-68.
  23. Mirhafez SR, Hariri M. L-arginine effect on inflammatory mediators: A systematic review of randomized controlled clinical trials. Int J Vitam Nutr Res. 2021;91(5-6):562-70.
  24. Dimitriou L, Sharp NC, Doherty M. Circadian effects on the acute responses of salivary cortisol and IgA in well trained swimmers. Br J Sports Med. 2002;36(4):260-4.
  25. Stewart L. Flynn MG, Campbell WW, Craig BA, Robinson JP, Timmerman KL, McFarlin BK, Coen PM, Talbert E. The influence of exercise training on inflammatory cytokines and C-reactive protein Med Sci Sports Exerc. 2007;39:1714-9.
  26. Bahmanbeglou NA, Ebrahim K, Maleki M, Nikpajouh A, Ahmadizad S. Short-duration high-intensity interval exercise training is more effective than long duration for blood pressure and arterial stiffness but not for inflammatory markers and lipid profiles in patients with stage 1 hypertension J Cardiopulm Rehabil Prev. 2019;39(1):50-5.
  27. Ahmadizad S, Avansar AS, Ebrahim K, Avandi M, Ghasemikaram M. The effects of short-term high-intensity interval training vs. moderate-intensity continuous training on plasma levels of nesfatin-1 and inflammatory markers. Horm Mol Biol Clin Investig. 2015;21(3):165-73.
  28. Banitalebi E, Kazemi A, Faramarzi M, Nasiri S, Haghighi MM. Effects of sprint interval or combined aerobic and resistance training on myokines in overweight women with type 2 diabetes: A randomized controlled trial. Life Sci. 2019;217:101-9.
  29. Schulte DM, Muller N, Neumann K, Oberhauser F, Faust M, Gudelhofer H, et al. Pro-inflammatory wnt5a and antiinflammatory sFRP5 are differentially regulated by nutritional factors in obese human subjects. PLoS One. 2012;7(2):e32437.
  30. do Nascimento MA, dos Santos Lira F, Punaro GR, de Mello MT, Tufik S, Higa EMS. Short-term l-arginine supplementation attenuates elevation of interleukin 6 level after resistance exercise in overweight men. Clin Nutr ESPEN. 2017;22:43-7.
  31. Hiratsu A, Tataka Y, Namura S, Nagayama C, Hamada Y, Miyashita M. The effects of acute and chronic oral l-arginine supplementation on exercise-induced ammonia accumulation and exercise performance in healthy young men: A randomised, double-blind, cross-over, placebo-controlled trial. J Exerc Sci Fit. 2022;20(2):140-7.
  32. Mohammadkhani PG, Irandoust K, Taheri M, Mirmoezzi M, Baić MJBRR. Effects of eight weeks of aerobic exercise and taking caraway supplement on C-reactive protein and sleep quality in obese women. Biol Rhythm Res. 2021;52(2):218-26.
  33. Bani Asadi M , Sharifi H , Abedi B, Fatolahi H. Acute inflammatory response to a single bout of resistance exercise with or without blood flow restriction. Int J Sport Stud Hlth. 2020;3(2):e110594.
  34. Gibson AL, Wagner D, Heyward V. Advanced Fitness Assessment and Exercise Prescription, 8E: Human kinetics; 2018.
  35. Asadi A, Ghasemi M, Zarandi E, Khanjari M M, Bayat S, et al. Effects of combined resistance-aerobic training and milk consumption on the weight loss of overweight female students. Int J Sport Stud Hlth.2(2):e97303.
  36. Lucotti P, Monti L, Setola E, La Canna G, Castiglioni A, Rossodivita A, et al. Oral L-arginine supplementation improves endothelial function and ameliorates insulin sensitivity and inflammation in cardiopathic nondiabetic patients after an aortocoronary bypass. Metabolism. 2009;58(9):1270-6.
  37. Bolboli L, Ghafari G, Rajabi A. Effect of omega-3 consumption and participate in aerobic exercise on sICAM-1 and proinflammatory cytokines in obese elderly women. Sport Physiology. 2014;6(21):79-94.
  38. Rosa Neto JC, Lira FS, Oyama LM, Zanchi NE, Yamashita AS, Batista ML, Jr., et al. Exhaustive exercise causes an anti-inflammatory effect in skeletal muscle and a proinflammatory effect in adipose tissue in rats. Eur J Appl Physiol. 2009;106(5):697-704.
  39. Febbraio MA, Steensberg A, Starkie RL, McConell GK, Kingwell BA. Skeletal muscle interleukin-6 and tumor necrosis factor-α release in healthy subjects and patients with type 2 diabetes at rest and during exercise. Metabolism. 2003;52(7):939-44.
  40. Monzillo LU, Hamdy O, Horton ES, Ledbury S, Mullooly C, Jarema C, et al. Effect of lifestyle modification on adipokine levels in obese subjects with insulin resistance. Obes Res. 2003;11(9):1048-54.
  41. Gibala MJ, Little JP, Macdonald MJ, Hawley JA. Physiological adaptations to low-volume, high-intensity interval training in health and disease. J Physiol. 2012;590(5):1077-84.
  42. Whyte LJ, Gill JM, Cathcart AJ. Effect of 2 weeks of sprint interval training on health-related outcomes in sedentary overweight/obese men. Metabolism. 2010;59(10):1421-8.
  43. Tremblay A, Simoneau JA, Bouchard C. Impact of exercise intensity on body fatness and skeletal muscle metabolism. Metabolism. 1994;43(7):814-8.
  44. Martina V, Masha A, Gigliardi VR, Brocato L, Manzato E, Berchio A, et al. Long-term N-acetylcysteine and L-arginine administration reduces endothelial activation and systolic blood pressure in hypertensive patients with type 2 diabetes. Diabetes Care. 2008;31(5):940-4.
  45. Hadiono M, Kushartanti BW, editors. High intensity interval training (HIIT) and moderate intensity training (MIT) against TNF-α and IL-6 levels in rats. 2nd International Conference on Sports Sciences and Health 2018 (2nd ICSSH 2018); 2019: Atlantis Press.
  46. Rahimi Saghand M, Rajabi H, Dehkhoda M, Hosseini A. The Effects of eight weeks high-intensity interval training vs. continuous moderate-intensity training on plasma dickkopf-1 and glycemic control in patients with type 2 diabetes. Ann-ApplSport-Sci. 2020;8(2):0-.
  47. Nawrocka A, Mynarski W. Objective assessment of adherence to global recommendations on physical activity for health in relation to spirometric values in nonsmoker women aged 60-75 years. J Aging Phys Act. 2017;25(1):123-7.
  48. Behrad A, Askari R, Hamedinia MR. The effect of high intensity interval training and circuit resistance training on respiratory function and body composition in overweight females. JPSBS.2016;4(7):89-101.
  49. Astorino TA, Schubert MM, Palumbo E, Stirling D, McMillan DW. Effect of two doses of interval training on maximal fat oxidation in sedentary women. Med Sci Sports Exerc. 2013;45(10):1878-86.
  50. Najafi M, Fatolahi H. The Effect of resistance training and vitamin d on leptin and hdl-c in overweight women. Int J Sport Stud Hlth.3(1):e104742.
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